Analytical Chromatography Laboratory

Laboratory Supervisor and Device Users

Kenan TURKAN

(Laboratory Supervisor and Device User)

E-Mail: kturkan@adiyaman.edu.tr 
Web: - 
Phone: +90 416 2233800 - 2354

Erkan DOGAN
(Device User)

E-Mail: erkandogan@adiyaman.edu.tr

Web: Erkan DOGAN
Phone: +90 416 2233800 - 2258

Device Name

Liquid Chromatography - Mass Spectrometry / Mass Spectrometer (LC-MS/MS) 

Device Brand and Model

SHIMADZU - 8040

General Information

LC / MS, HPLC (High Performance Liquid Chromatography) and MS (Mass Spectrometer) units are a device used to determine the content of the structure and the amount of the substances in the content by operating together. In the high performance liquid chromatography system, samples are analyzed by mass detector according to their physicochemical properties. Molecules ionize at the ionization source and pass to the mass spectrometer. The major ions formed in the first mass spectrometer are determined according to the m/z (mass/charge) ratio. This ion is broken down by collision gas (Nitrogen) in the collision cell and the ions formed as a result of the fragmentation are separated according to m/z (mass/charge) ratios in the second mass spectrometer. It is used for the quantitation of trace amounts of analytes in the determination of multiple analytes with high sensitivity and precision, with data on fragmentation ions.

Sample Preparation

Preparation of homogenized samples is made with Quechers kits.

Usage Area

• Qualitative and quantitative analysis of various samples,
• Pesticide, aflatoxin analysis in food samples,
• Investigation of DNA damage in tissue

Analysis Request Form for LC-MS / MS Analysis

Device Users

Kenan TURKAN

(Device User)

E-Mail: kturkan@adiyaman.edu.tr 
Web: - 
Phone: +90 416 2233800 - 2354

Mehmet KARAKUS
(Device User)

E-Mail: mehmetkarakus@adiyaman.edu.tr 
Web: - 
Phone: +90 416 2233800 - 2254    

Bayhan KARABULUT
(Device User)

E-Mail: bkarabulut@adiyaman.edu.tr
Web: Bayhan KARABULUT 
Phone: +90 416 2233800 - 2258    

 Device Name

High Pressure (Performance) Liquid Chromatography (HPLC) 

 Device Brand and Model

 SHİMADZU & Prominence LC-20A

General Information

High performance liquid chromatography (HPLC) is a special application of liquid chromatography method. As a result of reducing the particle size of the fillers used as stationary phase, the surface area of ​​the stationary phase interacting with the mobile phase is increased. Thus, the efficiency of the column is increased.. Pressure must be applied in order for the mobile phase to pass through column at a certain speed. HPLC is considered an indispensable tool in many fields today. The device is used to separate and identify species in a variety of organic, inorganic and biological samples. and is used to separate and identify species in various organic, inorganic and biological samples.

Sample Preparation

The sample to be analyzed in the HPLC device must be brought into liquid form. Samples' preparation varies according to the analysis to be made on the device. Samples are firstly pre-treated using organic solvents such as methanol, ethanol, cloroform, acetonitrile. After the preliminary processes are completed and the samples turned into liquid form are filtered, analysis is performed on the device

Usage Area

• Medicines (Antibiotics, sedatives, steroids, analgesics)
• Biochemicals (Amino acids, proteins, carbohydrates, lipids)
• Foodstuffs (Artificial sweeteners, antioxidants, aflatoxins)
• Industrial chemicals (Multi-ring aromatics, surfactants, propellants, dyes)
• Pollutants (Pesticides, herbicides, phenols, PCBs)
• Clinical medicine (Bile acids, drug metabolites, urea extracts, estrogens)
• Drugs (Drugs, poisons, blood alcohol, narcotics)

Analysis Request Form for HPLC Analysis

Device Users

Kenan TURKAN

(Device User)

E-Mail: kturkan@adiyaman.edu.tr 
Web: - 
Phone: +90 416 2233800 - 2354

Bayhan KARABULUT
(Device User)

E-Mail: bkarabulut@adiyaman.edu.tr
Web: Bayhan KARABULUT 
Phone: +90 416 2233800 - 2258

Fikret BUYUKKAYA KAYIS

(Device User)

E-Mail: fkayis@adiyaman.edu.tr  
Web: Fikret BUYUKKAYA KAYIS
Phone: +90 416 2233800 - 2253

Device Name

Gas Chromatography- Mass Spectrometry (GC-MS)

Device Brand and Model

SHIMADZU – QP 2010

General Information

GC-MS is the device used for determination and structure analysis of the substances separated in the gas chromatography column. While GC separates and ionizes the gases in the mixture from each other, MS determines these substances depending on their mass. There is also a flame ionization detector (FID) integrated into this system. GC / MS consists of five parts: injection unit, column, detector, column oven and auto sampler.

 Sample Preparation

Samples are firstly pre-treated using organic solvents. After the preliminary processes are completed and the samples turned into liquid form are filtered, analysis is performed on the deviceIn mass detector analysis  homogenized samples are prepared for analysis with Quechers kits. Different laboratory method is used for sample preparation in FID analysis.

Usage Area

• Food etc. pesticide analysis in samples,
• PCB analysis in environmental samples,
• Fatty acids and essential fatty acids analysis in FID detector

Analysis Request Form for GC-MS Analysis